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| Product Name | Pro-ISG15 protein |
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| Description | Interferon-stimulated Gene 15 (ISG15), also known as UCRP, is a ubiquitin-like protein that is covalently coupled to target proteins in a process termed ISGylation. It is a 165 amino acid (aa) polypeptide with a predicted molecular weight of 18 kDa. ISG15 exhibits 66% aa sequence identity with its mouse ortholog. Structurally, ISG15 consists of two tandem ubiquitin-like domains that share a similar 3-dimensional structure with ubiquitin and other ubiquitin-like modifiers including NEDD8 and SUMO1. Modification of targets by ISG15 occurs in a stepwise enzymatic process similar to that of ubiquitin. Enzymes regulating ISGylation include the activating (E1) enzyme UBE1L, the conjugating (E2) enzyme UbcH8, and ligases (E3) such as TRIM25 and HERC5. Removal of ISG15 is catalyzed by the deconjugating enzyme UBP43/USP18. Functionally, ISG15 has putative roles in the immune response and tumorigenesis. This is reflected by intracellular ISG15 targets that include Cyclin D1, tumor suppressor p63, IRF3 and a range of viral proteins. It is induced by type 1 interferons and microbial infection and knockout mice exhibit an increased sensitivity to infection by some viruses. ISG15 can also be secreted by cells of the immune system and may act in a cytokine-like manner. For instance, it is produced by human granulocytes in response to mycobacterium exposure and natural killer cells and T cells respond to extracellular ISG15 with IFN-gamma production. Further supporting a role in immune function, ISG15 mutations are associated with MSMD, an inherited disorder characterized by increased susceptibility to mycobacterial infection. Pro-ISG15 (165 amino acids) is the inactive precursor of ISG15 (163 amino acids). The precursor is processed at the C-terminus by an ISG15-specific protease UBP43 (also knownas USP18). The mature form of ISG15 contains the conserved C-terminal di-glycine residues which are critical in activation and conjugation reactions. |
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| Size | 500 µg |
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| Concentration | n/a |
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| Applications | SDS-PAGE (SDS) |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | ABIN2745806 |
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| Order / More Info | Pro-ISG15 protein from ANTIBODIES-ONLINE GmbH |
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| Product Specific References | n/a |
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