| 产品详情 |
| Edit |   |
|---|
| Product Name | Ubiquitin-AMC Substrate, Fluorogenic (Ubiquitin-aminomethylcoumarin) |
|---|
| Description | Purity ~95% (HPLC). Prepared by the C-terminal derivatisation of ubiquitin with 7-amino-4-methylcoumarin. Acts as a useful and sensitive fluorogenic substrate for a wide range of deubiquitinylating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs). Ubiquitin-AMC has been shown to be a sensitive substrate for UCH-L3 (Km=0.039uM) and for Isopeptidase-T (Km=0.17-1.4uM), as well as for studying deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential. Sequence: C-terminal derivatisation of ubiquitin with 7-amino-4-methylcoumarin Emission: 460nm Excitement: 380nm Molecular Weight: ~8.6kD Substrate Concentration: 0.01-1uM Enzyme Concentration: 10-100pM (UCH-L3); 10-100nM (Isopeptidase-T) Applications: Suitable for use in Deubiquitinylating Enzyme Activity. |
|---|
| Size | 25ug |
|---|
| Concentration | n/a |
|---|
| Applications | n/a |
|---|
| Other Names | n/a |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | U0999-07 |
|---|
| Price | |
|---|
| Order / More Info | Ubiquitin-AMC Substrate, Fluorogenic (Ubiquitin-aminomethylcoumarin) from UNITED STATES BIOLOGICAL |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|