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Antigenic Specificity | Green Fluorescent Protein (GFP) (N-Term) |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | Aequorea victoria |
Isotype | n/a |
Format | antigen affinity purified |
Size | 0.1 mg |
Concentration | n/a |
Applications | Immunohistochemistry (IHC) |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | GFP(Green fluorescent protein) is an energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. It contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. |
Immunogen | Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to N-terminal residues of Aequorea victoria GFP (Green fluorescent protein) |
Other Names | n/a |
Gene, Accession # | n/a |
Catalog # | ABIN966201 |
Price | |
Order / More Info | Green Fluorescent Protein (GFP) (N-Term) Antibody from ANTIBODIES-ONLINE GmbH |
Product Specific References | PubMed: 1347277, 3858813, 8137953, 8448132, 8703075, 9782051, 9631087 |