| 产品详情 |
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| Product Name | Uracil DNA Glycosylase (UNG) |
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| Description | Catalyzes the hydrolysis of N-glycosylic bond between the uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. It shows no activity on RNA (1). Unit Definition: 1unit of Uracil DNA Glycosylase catalyses the release of 1nanomole of uracil from uacil containing DNA template in 60 min at 37°. Storage Buffer: Supplied as a liquid in 30mM Tris-HCl, pH 7.5, 150mM sodium chloride, 1mM EDTA, 1mM DTT, 0.05% Tween 20, 50% glycerol. 10X Reaction Buffer U1900-05A): Supplied as a liquid in 200mM Tris-HCl, pH 8.2, 10mM EDTA, 100mM sodium chloride. Applications: Suitable for GMPD detection, Site-directed mutagenesis (2), as a Probe for protein-DNA interaction (3), Rapid, efficient cloning of PCR products (4) anddH2Oelimination of carry over contamination in PCR. Note: The abasic sites formed in DNA by uracil-DNA glycosylase may be cleaved by heat under alkaline conditions. Any of the five USBio buffers can be used instead of 10X uracil-DNA glycosylase rea |
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| Size | 200U, 1000U |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | Supplied with 10X Reaction buffer |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | U1900-05 |
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| Order / More Info | Uracil DNA Glycosylase (UNG) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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