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| Product Name | Glutamate Dehydrogenase, NADP |
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| Description | Purified. Glutamate Dehydrogenase, NADP is useful for enzymatic determination of NH3, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease (URH-201) in clinical analysis. L-Glutamate + H20 + NADP+ = 2-Oxoglutarate + NH3 + NADPH Activity: ≥4,000 units/ml Protein Content: ≥10mg/ml Unit Definition: One unit will reduce 1umole of α-ketoglutarate to L-glutamate per min at pH 8.3 at 30°C in the presence of ammonium ions and NADPH. Isoelectric Point: 4.6 Michaelis Constants: 1.1x10e-3M (NH3), 3.4x10e-4M (α-Ketoglutarate) 1.2x10e-3M (L-Glutamate), 1.4x10e-5M (NADPH), 1.5x10e-5M (NADP+) Structure: 6 subunits (M.W.50,000) per mol of enzyme Inhibitors: Hg++, Cd++, p-chloromercuribenzoate, pyridine, 4-4-dithiopyridine, 2,2-dithiopyridine Optimum pH: 8.5 (α-KG→L-Glu) 9.8 (L-Glu→α-KG) Optimum Temperature: 45°C(α-KG−L-Glu) 45-55°C (L-Glu→α-KG) pH Stability: pH 6.0-8.5 (25°C, 20hr) The |
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| Size | 1KU, 5KU |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | EC 1.4.1.4; GDH, GLDH, GLUD 1, GLUD, GLUD1, GLUD1, Glutamate Dehydrogenase 1, Glutamate Dehydrogenase 1 Mitochondrial, MGC132003; Glutamic dehydrogenase; glutamate (nicotinamide adenine dinucleotide (phosphate)); Glutamic acid dehydrogenase; L-Glutamate dehydrogenase; NAD(P)-Glutamate dehydrogenase; NAD(P)H-dependent Glutamate dehydrogenase; Glutamate dehydrogenase (NADP) |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | G4000-50B1 |
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| Order / More Info | Glutamate Dehydrogenase, NADP from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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