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| Product Name | PARP1, Chemiluminescent, BioAssay™ Kit (Poly Polymerase 1, Poly Transferase, ADP-Ribose, ADPRT) |
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| Description | PARP1 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones. Intended Use: The PARP1 Chemiluminescent Activity BioAssay™ kit is designed to measure PARP1 activity for screening and profiling applications. Test Principle: The key to the PARP1 Chemiluminescent Activity BioAssay™ is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with the assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce a chemiluminescence that can then be measured using a chemiluminescence reader. This kit is also suitable for use with cell extracts, but nonspecific ribosylation activity may be detected. Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt Applications: Great for studying enzyme kine |
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| Size | 96 tests |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 298438 |
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| Order / More Info | PARP1, Chemiluminescent, BioAssay™ Kit (Poly Polymerase 1, Poly Transferase, ADP-Ribose, ADPRT) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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