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| Product Name | HIF-1 Alpha DNA Binding Activity Assay Kit |
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| Description | The HIF-1 Alpha DNA Binding Activity Assay Kit is an ELISA for the measurement of activated HIF-1. A biotinylated double stranded DNA containing an HRE is first bound to the provided Streptavidin plate wells. Then protein samples are added to the DNA conjugated microplate. After a brief incubation, an anti-HIF-1 Alpha antibody is added, followed by an HRP conjugated secondary antibody, allowing for simple colorimetric detection by plate spectophotometry. Background: Mammalian cells are able to s |
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| Size | 96 assays |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | MBS168165 |
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| Order / More Info | HIF-1 Alpha DNA Binding Activity Assay Kit from MYBIOSOURCE INC. |
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| Product Specific References | 1. Ke Q and Costa M. (2006) Molecular Pharm. 70:1469-1480.2. Salceda S and Caro J (1997) J. Biol. Chem. 272: 22642-22647.3. CW Pugh, JF O'Rourke, M Nagao, JM Gleadle, and PJ Ratcliffe (1997) J. Biol. Chem. 272: 11205-11214.4. LE Huang, Z Arany, DM Livingston, and HF Bunn (1996) J. Biol. Chem. 272: 32253-32259.5. PJ Kallio, I Pongratz, K Gradin, J McGuire, and L Poellinger (1997) Proc Natl Acad Sci USA 94: 5667-5672 |
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