| 产品详情 |
| Edit |   |
|---|
| Product Name | Normal Rat Kidney Whole Cell Lysate |
|---|
| Description | Ready-to-use whole cell lysates produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins. |
|---|
| Size | 500 µg |
|---|
| Concentration | 1.0 mg/mL |
|---|
| Applications | WB. Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 µl depending on the size format of your gel. |
|---|
| Other Names | Normal Rat Kidney Whole Cell Lysate, Rat Kidney WCL, Rat Kidney Lysate |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | W12-000-T090 |
|---|
| Price | |
|---|
| Order / More Info | Normal Rat Kidney Whole Cell Lysate from ROCKLAND IMMUNOCHEMICALS INC. |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|