ADAM MC自动细胞计数仪

哺乳动物细胞的准确计数

与FACS计数一样准确
您是否正在寻找一种自动化的计数器来达到准确性的顶峰？许多实验室已经将ADAM MC作为一种解决方案，可以像流式细胞仪一样产生高度可重复的结果，但成本却很少。它的技术很简单；它结合了精密显微镜，集成的荧光检测光学系统和机载分析软件。使用使用碘化丙啶染色靶细胞核的基于芯片的一次性试剂盒，ADAMwu需担心细胞的大小，形状或聚集。取而代之的是，它使用荧光来对染色的细胞核进行专门成像，并且每个样品的平均22个立读数总计3 ?l读数体积。这些改进专门解决了由于样品读数低（某些竞争对手的模型仅执行一次读数），样品体积低（大多数模型的读数为1 ?l或更少）和细胞分辨率不准确（通常在细胞聚集在一起时发现）的差异。当R2值超过0.99时，ADAM MC可以使用碘化丙啶技术直接替代手动细胞计数。结果是惊人的，大多数细胞系的CV可能低于10％。 ADAM MC可以处理各种类型的悬浮细胞，因为仅需检测细胞核即可，并且使用非常聪明的“洗涤剂洗涤”技术来提供j确的细胞活力值。但是请不要相信我们-我们邀请您要求演示并亲自找到。

即使是本科生也可以使用它
我们是否提到过ADAM MC的设计易于使用？其一次性芯片组大程度地减少了污染风险，大大简化了工作流程，并且wu需进行校准和定期清洁。其板载分析wu需使用外部计算机，因此可以快速进行操作。后，ADAM MC非常小，可以舒适地坐在任何台式机上，即使是本科生也可以

COMPARISON WITH FLOW CYTOMETRY

Comparison of cell viabilities between the ADAM and flow cytometry. SK-OV, HeLa and NCI-H23 cells
were treated 100 and 300?M H2O2 for 3 hours and then analyzed by the ADAM and flow cytometry.
Values are given as ± SD of three experiments.

HEMOCYTOMETER AND ADAM CLOSELY CORRELATE

These results show that with propridium iodide-stained CHO cells the ADAM MC counts are nearly identical to manual counting using a C-Chip Disaposable Hemocytometer. In practice, greater variability is possible because a glass hemocytometer cover slip floats, leading to wider variation in counting volumes. Additionally, trypan blue and propidium iodide stain different components of the cells. Propidium iodide and trypan blue counts typically vary by less than 5%.

CONTROL SOLUTIONS WITH LOW, MEDIUM AND HIGH CONCENTRATIONS OF FLUORESCENT BEADS WERE COUNTED WITH THE ADAM

Typical results with actual cell samples will vary between 5% and 20% CV.

THE ADAM IS BASED ON STAINING MAMMALIAN CELL DNA WITH A FLUORESCENT DYE, PROPIDIUM IODIDE (PI).

The ADAM is based on staining mammalian cell DNA with a fluorescent dye, Propidium Iodide (PI). PI does not enter cells with intact cell membranes or active metabolism. In contrast, cells with damaged membranes or with inactive metabolism are unable to prevent PI entering the cell. As a result, the nuclei of non-viable cells will only be stained. The Adam provides two kinds of 1-2. Technology – Viability Measurement staining solutions. AccuStain Solution T for the total cell counting is composed of the fluorescent dye (PI) and lysis solution. AccuStain Solution N for the non-viable cell counting is composed of the fluorescent dye and PBS. In order to measure the total concentration of cells, the plasma membranes of all the cells must be disrupted to stain all the Nuclei with PI. The process of disrupting and staining is achieved by treatment with AccuStain Solution T. In the second solution, live cells remain intact and are not stained. Only the non-viable cells are stained and detected. After treatment, the prepared cells will be loaded into the chip. The viability will be automatically calculated in the Adam-MC software after each measurement of the total cells and the non-viable cells.

SPECIFICATIONS
Light Source	Green LED (532nm)
Objective Lens	4x
Camera	CCD
Dynamic Range	5 x 104 – 4 x 106 cells/ml
Analysis Time	45s – 1.5 min/test
Sample Volume	12 ?l /test
Measuring Volume	3 ?l/test
Tests Per Chip	400 – Cell viability 800 – Total cell count
Cell Type	Suspension cells
Cell Size	Any size
Part Numbers	DBA01, DBA01K, ADR-1000
Detection Method	Stained cell nucleus
Power Requirements	100-240 VAC, 50-60 Hz
Weight	9 kg
Size	220 x 375 x 250 mm
Warranty	1 year parts and labor

二、DBA01K – ADAM MC AccuChip 4x Kit w/AccuStain T/N Solution (400 tests – cell viability, 800 tests – total cell count)

三、ADR-1000 – Extra AccuStain T/N Solution (50 ml & 25ml)

四、ADB-500 – ADAM Calibration Beads – Standard Bead Solution (~1 x 10E6/ml)