3DProSeed ® Hydrogel Well - Plate

Pre-assembled synthetic hydrogels in a microtiter plate for ready-to-use and automation-compatible  cell-based assays in 3D. The patented 3DProSeed®  hydrogel surface enhances the penetration of cells.

No hydrogel assembly step

The hydrogels in the plate are hydrated

ready for cell seeding

Fully synthetic

poly(ethlyene glycol)-based

For the highest control over the culture conditions

Sequential Seeding

of various cell populations at different time points

of your assay for complex co-cultures

KEY FEATURES

3DProSeed® Hydrogel Plate

Our 3DProSeed® microtiter plate combines the advantages of hydrogel-based cultures with the simplicity and workflow integration of standard polystyrene cultureware.

 

Our plate contains pre-assembled synthetic hydrogels, delivered to you hydrated, ready for cell seeding.

 

The hydrogels are fully synthetic made of state-of-art poly(ethlyene glycol)-based bioconjugates. This hydrogel material offers the highest level of control over the culture conditions and optical transparency for high quality microscopy.

The key innovation is the 3DProSeed hydrogel surface featuring an in-depth density gradient which enables penetration of seeded cells into the bulk.

Our special surface makes it possible to sequentially seed various cell populations in the same gel to establish complex co-cultures.

SPECIFICATIONS

1. The plate

Standard ANSI footprint 96-well black imaging plate with 180 microns glass bottom.

Plate sealed with a polyproylene adhesive foil and covered with its standard polystyrene lid.

 

 

 

2. The Hydrogel

Pre-assembled hydrogels at the bottom of each well. Hydrogels delivered hydrated in a storage buffer ready for cell seeding.The special inner-well geometry for facilitated manual pipetting.

Optically clear synthetic hydrogels made of poly(ethylene glycol) bio-conjugates.

Basic formulation contains cell adhesion sites and protease-sensitive degradation sites. Advanced formulations are available depending on the application of interest.

3. Patented hydrogel in-depth density gradient

The hydrogel gradually disappears in the proximity of the upper surface becoming water.

This special surface enhances penetration of cells seeded and enables repeated seeding of various cell populations for the development of advanced co-culture models in the gel.

Request plate dimensions

RESEARCH APPLICATIONS

Oncology and Immuno-oncology

Highlights

Tumor cells cultured in a pre-established stromal microenvironment made of fibroblasts, mesenchymal stromal cells, adipocytes or endothelial cells.

Sequential addition of immune cells to complex tumor cultures for killing assays and IO studies.

Learn More

Neurosciences

Highlights

Culture of human astrocytes, human progenitors and iPSCs-derived neurons and neuronal differentiation in the gel.

 

Co-cultures of iPSC-neurons with astrocytes and microglia via sequential seeding.

Phenotypic assays on microglia monocultures.

Learn More

Stem Cells Culture and Differentiation

Highlights

Culture of human primary mesenchymal stem cells.

Adipogenic and osteogenic differentiation.

Learn More

Angiogenesis 

Highlights

Culture of human primary mesenchymal stem cells with endothelial cells.

Interaction of cancer cells with the vascular niche.